multiple tissue northern blot analysis multiple normal human tissue blots mnhtb  (TaKaRa)

Journal: American journal of physiology. Gastrointestinal and liver physiology

Article Title: Function, expression, and characterization of the serotonin transporter in the native human intestine

doi: 10.1152/ajpgi.00354.2007

Figure Lengend Snippet: Northern blots of poly(A)+ RNA from different human tissues were probed with a 491-bp fragment of human SERT cDNA (A). Arrows indicate RNA size markers (in bp). Higher exposure of ileum lane is shown in B.

Article Snippet: To ascertain the presence of mRNA species encoding SERT in various human intestinal tissues, commercially available human multiple-tissue Northern blots (BioChain) were probed with 32 P-labeled 491-bp human SERT cDNA fragment.

Techniques: Northern Blot

Journal: PLoS ONE

Article Title: Identification of MAMDC1 as a Candidate Susceptibility Gene for Systemic Lupus Erythematosus (SLE)

doi: 10.1371/journal.pone.0008037

Figure Lengend Snippet: Northern blot analysis of MAMDC1 mRNA expression in different human tissues, showing several expressed splice variants. MAMDC1 mRNA transcript corresponding to the full-length isoform is indicated by an arrowhead on the right side. A β-Actin cDNA control probe was used for normalization (bottom).

Article Snippet: Fifty nano grams (ng) of purified cDNA were then labelled with P 32 -dCTP (GE Healthcare, Buckinghamshire, UK) by random priming and was used to probe two human multiple tissue polyA+ RNA Northern blots (HB2010 and HB2011, OriGene Technologies) according to manufacturer's instructions.

Techniques: Northern Blot, Expressing, Control

Journal: PLoS ONE

Article Title: Identification of MAMDC1 as a Candidate Susceptibility Gene for Systemic Lupus Erythematosus (SLE)

doi: 10.1371/journal.pone.0008037

Figure Lengend Snippet: THP-1 cells were treated for 24 h with LPS, TGF-β1, IL-1β, IFN-γ, TNF-α, or a combination of TNF-α and IFN-γ, and MAMDC1 mRNA expression was quantified by Real-Time PCR in triplicate experiments. The results are reported as fold changes relative to THP-1 cells grown in the absence of stimulation (control), with the smallest observation, lower quartile, median, upper quartile, and largest observation shown for each sample.

Article Snippet: Fifty nano grams (ng) of purified cDNA were then labelled with P 32 -dCTP (GE Healthcare, Buckinghamshire, UK) by random priming and was used to probe two human multiple tissue polyA+ RNA Northern blots (HB2010 and HB2011, OriGene Technologies) according to manufacturer's instructions.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control